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A novel nanoprotein particle synthesis: Nanolipase

Paper ID Volume ID Publish Year Pages File Format Full-Text
35267 45083 2011 5 PDF Available
Title
A novel nanoprotein particle synthesis: Nanolipase
Abstract

Lipases (triacylglycerol acylhydrolases; E.C. 3.1.1.3) constitute a group of enzymes defined as carboxylesterases that catalyse the hydrolysis (and synthesis) of long-chain acylglycerols at the lipid–water interface. In this study, a novel method has been developed to prepare nanoprotein particles carrying lipase using a photosensitive microemulsion polymerization process. The nanostructured lipases with photosensitive features have been characterized by transmission electron microscopy (TEM) and Zeta Sizer. The average particle size of nanolipases was found to be about 100 nm. Lipase nanoparticles were used in hydrolysis of paranitrophenyl palmitate (p-NPP) and the results were compared with free lipase. The parameters like pH, temperature and hydrolytic activity that affect p-NPP hydrolysis were investigated by using lipase nanoparticles and compared to free lipase. Lastly, reusability of lipase nanoparticles was investigated and according to our results, this novel lipase nanoparticles showed admirable potential in reusable catalyst.

Keywords
Nanolipase; Photosensitive microemulsion polymerization; Photosensitive cross-linking; Proteinous polymeric particles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 8, August 2011, Pages 1688–1692
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us