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A highly efficient diastereoselective synthesis of α-isosalicin by maltase from Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
35269 45083 2011 5 PDF Available
Title
A highly efficient diastereoselective synthesis of α-isosalicin by maltase from Saccharomyces cerevisiae
Abstract

In this report, α-isosalicin, a potent anticoagulant and skin whitening agent, was synthesized by a highly efficient chemoselective and diastereoselective reaction, catalyzed by maltase from bakers’ yeast (Saccharomyces cerevisiae). The highest yield of this one-step transglucosylation reaction was achieved with 50 mM of salicyl alcohol as a glucose acceptor. The key reaction factors were optimized using response surface methodology (RSM) with an enzyme concentration of 10 U/mL. The optimum temperature of the reaction was determined as 36.5 °C, the optimal maltose concentration was 40% (w/v), the optimal pH was 6.5, and the optimal reaction time was 16 h. Under these conditions 75% of α-isosalicin was obtained, with a yield of 10 g/L, and no by product formation was observed.

Keywords
α-Isosalicin; Glucosidase; Salicyl alcohol; Transglucosylation; Bakers’ yeast
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A highly efficient diastereoselective synthesis of α-isosalicin by maltase from Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 8, August 2011, Pages 1698–1702
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us