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Separation of phenylalanine and tyrosine by ion-exchange using a strong-base anionic resin. II. Cyclic adsorption/desorption studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
3527 173 2012 10 PDF Available
Title
Separation of phenylalanine and tyrosine by ion-exchange using a strong-base anionic resin. II. Cyclic adsorption/desorption studies
Abstract

Cyclic adsorption and desorption dynamics of phenylalanine (Phe) and tyrosine (Tyr) was investigated in a column packed with a strong anionic resin, Diaion PA316, under different pH conditions. Experiments were performed in order to study the effect of the pH in two sequential bed saturations keeping constant the feed concentration. A adsorption step at isoelectric pH (≈6) followed by a desorption step at high pH (12–13) causes an overshoot in the breakthrough curves due to lower affinity resin by amino acid under alkaline conditions. Contrarily to this behaviour, the solution that leaves the column reaches a zero concentration of amino acid during a certain time in two consecutive saturations at high pH and isoelectric pH. The regeneration bed with 0.14 M NaOH is almost effective for conditions less favourable of sorption of amino acid by the resin. Satisfactory agreement was achieved between experimental and simulated results using a mathematical model that considers axial dispersed plug flow and intraparticle mass transfer described by the linear driving force model. Preliminary pH parametric pumping experiments, in semi-continuous mode, enabled to achieve a concentration factor of the top product of ≈2.3 and 2.0 for Phe and Tyr during 10 cycles of operation.

► Sequential bed saturations of Phe and Tyr were analysed under different pH values. ► Regeneration of resin loaded with amino acid at high pH is effective with 0.14 M NaOH. ► A top product enriched in amino acid was obtained via pH parametric pumping runs. ► Experimental data were suitably simulated with a model with linear driving force.

Keywords
Cyclic adsorption/desorption; Ion-exchange resin; Amino acids; Phenylanine and tyrosine separation; Modeling; pH parametric pumping
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Separation of phenylalanine and tyrosine by ion-exchange using a strong-base anionic resin. II. Cyclic adsorption/desorption studies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 67, 15 August 2012, Pages 241–250
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us