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A new conception of enzymatic membrane reactor for the production of whey hydrolysates with low contents of phenylalanine

Paper ID Volume ID Publish Year Pages File Format Full-Text
35273 45084 2009 8 PDF Available
Title
A new conception of enzymatic membrane reactor for the production of whey hydrolysates with low contents of phenylalanine
Abstract

A novel concept of enzymatic membrane reactor (EMR) is presented, aiming at the production of cheese whey protein hydrolysates with low contents of phenylalanine (Phe) for the diet of phenylketonuria patients. Whey proteins were first hydrolyzed by chimotrypsin, followed by the action of carboxypeptidase A (CPA), immobilized on agarose gel particles, which were retained inside the reactor using a filter. The liquid medium passes through hollow fiber ultrafiltration unit (1 kDa cut-off), and the retentate is recycled to the reactor. The innovation here is that the membrane is not used to retain the enzyme neither physically nor as an immobilization support. The EMR provided a higher performance than the classical, sequential approach: batch reaction followed by ultrafiltration. It was confirmed that the removal of products promoted by the EMR enhances reaction rates, due to the reduction of inhibition effects. A mathematical model of the EMR is also presented and validated.

Keywords
Cheese whey proteolysis; Enzymatic membrane reactor; Enzymatic proteolysis modeling; Carboxypeptidase A; Immobilized enzyme; PKU dietary concentrate
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A new conception of enzymatic membrane reactor for the production of whey hydrolysates with low contents of phenylalanine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 3, March 2009, Pages 269–276
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us