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Microbial reduction of ethyl acetoacetate to ethyl (R)-3-hydroxybutyrate in an ionic liquid containing system

Paper ID Volume ID Publish Year Pages File Format Full-Text
35280 45084 2009 6 PDF Available
Title
Microbial reduction of ethyl acetoacetate to ethyl (R)-3-hydroxybutyrate in an ionic liquid containing system
Abstract

A hydrophilic ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM]BF4) was successfully employed as co-solvent for asymmetric bioreduction of ethyl acetoacetate (EOB) to ethyl (R)-3-hydroxybutyrate (R-EHB) catalyzed by Pichia membranaefaciens Hansen ZJPH07 cells. The results demonstrated that the addition of [BMIM]BF4 in reaction system can markedly reduce the substrate inhibition and moderately improve the enantioselectivity compared to that in monophasic aqueous system. Among different alcohols and carbohydrates tried as co-substrate, glucose was a proper electron donor. Although isopropanol gave the best enantioselectivity with the highest yield, S-enantiomer was obtained. To optimize the bioreduction, some reaction parameters for the biosynthesis of R-EHB in this IL-containing system were investigated, such as temperature, buffer pH, shaking speed, substrate concentration, wet cells concentration and reaction time. Under the optimum conditions, best conversion of 77.8% and product enantiomeric excess (e.e.) of 73.0% were obtained. A comparative study was performed either in the presence or in the absence of [BMIM]BF4, higher reaction yield (77.8% versus 68.5%) and product e.e. (73.0% versus 65.1%) were observed in IL-containing system with 0.55 M of the substrate, but 0.35 M of substrate concentration for the reduction in aqueous system without the addition of [BMIM]BF4.

Keywords
Ionic liquid; Pichia membranaefaciens Hansen; Asymmetric bioreduction; Ethyl acetoacetate; Ethyl (R)-3-hydroxybutyrate
First Page Preview
Microbial reduction of ethyl acetoacetate to ethyl (R)-3-hydroxybutyrate in an ionic liquid containing system
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 3, March 2009, Pages 316–321
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering