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Effect of the degree of cross-linking on the properties of different CLEAs of penicillin acylase

Paper ID Volume ID Publish Year Pages File Format Full-Text
35281 45084 2009 5 PDF Available
Title
Effect of the degree of cross-linking on the properties of different CLEAs of penicillin acylase
Abstract

Cross-linked enzyme aggregates (CLEAs) are novel type biocatalysts well suited to catalyze reactions of organic synthesis. Penicillin acylase is a versatile enzyme that can both hydrolyze and synthesize β-lactam antibiotics. CLEAs and CLEAs covered with polyionic polymers (polyethyleneimine and dextran sulfate at two different enzyme to polymer ratios) were prepared at varying cross-linking agent to enzyme ratio: 0.15 and 0.25. Results are presented on the effect of such variables on immobilization yield, specific activity, stability and performance of penicillin acylase CLEAs in the kinetically controlled synthesis of cephalexin. The cross-linking agent to enzyme ratio had no significant effect on the specific activity of the CLEAs, but affected immobilization yield, stability in ethylene glycol medium and conversion yield and productivity in the synthesis of cephalexin, being always higher at the lower cross-linking agent to enzyme ratio. Best results were obtained with CLEAs at 0.15 glutaraldehyde to enzyme protein ratio: specific activity of hydrolysis and synthesis was 708 and 325 UI/gCLEA respectively, conversion yield was 87%, specific productivity was 5.4 mmol cephalexin/(gCLEA·h) and 90% of the enzyme remained active after 170 h at operating conditions.

Keywords
CLEAs; Enzyme stabilization; Penicillin acylase; Organic cosolvents; Cephalexin; Saline microenvironment
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Effect of the degree of cross-linking on the properties of different CLEAs of penicillin acylase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 3, March 2009, Pages 322–326
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us