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Biochemical and hydrolytic properties of multiple thermostable α-galactosidases from Streptomyces griseoloalbus: Obvious existence of a novel galactose-tolerant enzyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
35282 45084 2009 7 PDF Available
Title
Biochemical and hydrolytic properties of multiple thermostable α-galactosidases from Streptomyces griseoloalbus: Obvious existence of a novel galactose-tolerant enzyme
Abstract

The multiple α-galactosidases from Streptomyces griseoloalbus—α-Gal I, α-Gal II and α-Gal III were purified to homogeneity by a two-step chromatographic process. The molecular masses and pI of the three enzymes were 72, 57 and 35 kDa, and 4.41, 5.6 and 6.13, respectively. α-Gal I showed N-terminal sequence homology to S. coelicolor A3(2) family 27 α-galactosidase. The optimum pH and temperature of the three α-galactosidases were 5.0, 6.5 and 5.5 and 65, 50 and 55 °C, respectively. α-Gal I was stable up to 65 °C and α-Gal II and α-Gal III up to 55 °C for 2 h. Based on the hydrolytic properties α-Gal I could be classified as a member of GH27 family and α-Gal II and α-Gal III as members of GH36 family. Metal cations like Hg2+, Ag2+ and Cu2+ inhibited enzyme activity while Mg2+ enhanced the activity of α-Gal I. Interestingly α-Gal I showed unusual tolerance to even higher concentrations of galactose, unlike the other two α-galactosidases, which were competitively inhibited by galactose. Melibiose was a competitive inhibitor of all three enzymes. Histidine, tryptophan and carboxylic residues were essential for catalytic action of the three α-galactosidases.

Keywords
Streptomyces griseoloalbus; α-Galactosidase; Purification; Thermostability; Polymeric galactomannan hydrolysis; Galactose tolerance
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Biochemical and hydrolytic properties of multiple thermostable α-galactosidases from Streptomyces griseoloalbus: Obvious existence of a novel galactose-tolerant enzyme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 3, March 2009, Pages 327–333
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us