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Immobilization of antibodies through the surface regions having the highest density in lysine groups on finally inert support surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
35289 45084 2009 4 PDF Available
Title
Immobilization of antibodies through the surface regions having the highest density in lysine groups on finally inert support surfaces
Abstract

Immobilization of anti-horseradish peroxidase on glyoxyl-agarose proceeds rapidly, and after the immobilization, it was found that the antibody captured almost the same amount of peroxidase than the free antibody. After boiling the antibodies in the presence of SDS and mercaptoethanol, more than 95% of the immobilized antibodies presented the four subunits attached to the support.The reduction of the preparation converts the glyoxyl groups into very hydrophilic and inert hydroxyl-groups. That way, the final support was fully unable to adsorb any protein under any condition, and the only adsorbed proteins on the immobilized antibody are these recognized by the antibody. The immobilized antibody maintained intact their capacity to capture peroxidase after 20 weeks of storage at 4 °C.The high functionality of the immobilized antibody and the fully inert surface suggest that this technique may be a very suitable one to immobilize antibodies for biosensor design or immuno-chromatographic matrices.

Keywords
Immobilized antibodies; Glyoxyl supports; Oriented immobilization; Inert surfaces; Biosensor; Immuno-chromatography matrices
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Immobilization of antibodies through the surface regions having the highest density in lysine groups on finally inert support surfaces
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 3, March 2009, Pages 365–368
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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