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Batch operational stability of immobilized heterologous Rhizopus oryzae lipase during acidolysis of virgin olive oil with medium-chain fatty acids

Paper ID Volume ID Publish Year Pages File Format Full-Text
3529 173 2012 4 PDF Available
Title
Batch operational stability of immobilized heterologous Rhizopus oryzae lipase during acidolysis of virgin olive oil with medium-chain fatty acids
Abstract

Structured triacylglycerols containing medium-chain fatty acids (M) at positions sn-1,3 and long-chain fatty acids (L) at the sn-2 position (MLM type), were obtained by acidolysis of virgin olive oil with caprylic or capric acid, in solvent-free media, at 40 °C, catalyzed by a heterologous Rhizopus oryzae lipase (r-ROL) immobilized in Eupergit® C or in Lewatit VP OC 1600. The biocatalyst immobilized in Eupergit® was reused in consecutive 23-h batches with rehydration of the biocatalyst between batches. A first-order deactivation was observed (half-life time, t1/2 = 39.0 h for caprylic; t1/2 = 54.3 h for capric acid). During acidolysis with capric acid catalyzed by r-ROL immobilized in Lewatit VP OC 1600, without rehydration, a first-order deactivation was observed (t1/2 = 49.1 h); with rehydration, a considerable increase in stability was observed (t1/2 = 234 h; Sadana's series-type inactivation kinetics model).

► Rhizopus oryzae heterologous lipase (r-ROL) was immobilized in different supports. ► r-ROL was able to catalyze acidolysis of olive oil with caprylic or capric acid. ► r-ROL was reused, either without or with biocatalyst rehydration between batches. ► Operational stability of r-ROL in Lewatit VPOC 1600 increased with rehydration.

Keywords
Acidolysis; Biocatalysis; Enzyme deactivation; Heterologous Rhizopus oryzae lipase; Immobilization; Structured lipids
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Batch operational stability of immobilized heterologous Rhizopus oryzae lipase during acidolysis of virgin olive oil with medium-chain fatty acids
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 67, 15 August 2012, Pages 265–268
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us