fulltext.study @t Gmail

Effect of iron and aeration on superoxide dismutase and catalase activity of PHB-producing Azotobacter chroococcum

Paper ID Volume ID Publish Year Pages File Format Full-Text
35290 45084 2009 4 PDF Available
Title
Effect of iron and aeration on superoxide dismutase and catalase activity of PHB-producing Azotobacter chroococcum
Abstract

The effect of aeration level and iron concentration on Azotobacter chroococcum 23 growth, PHB accumulation and antioxidative enzyme activities was investigated in shake flask experiments. Biomass yield and carbon source conversation coefficients increased in the presence of iron in the growth medium and under decreased aeration. The highest biomass production was observed for the culture grown in a medium with 36 μM of initial iron concentration and moderate aeration level. The highest PHB accumulation level (70–72% from cell dry weight) under our experimental conditions was observed at decreased aeration in the growth medium with 180 μM of initial iron concentration. Results obtained prove that both aeration level and iron supply have a marked influence on the activity of SOD and catalase. Bearing in mind the necessity of iron for the synthesis of both enzymes, only catalase showed a specific dependence on the intracellular iron accumulation level.

Keywords
Azotobacter chroococcum 23; Poly-β-hydroxybutyrate (PHB); Iron accumulation; Oxygen; Catalase; Superoxide dismutase (SOD)
First Page Preview
Effect of iron and aeration on superoxide dismutase and catalase activity of PHB-producing Azotobacter chroococcum
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 3, March 2009, Pages 369–372
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering