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A putative secreted solute binding protein, SCO6569 is a possible AfsR2-dependent down-regulator of actinorhodin biosynthesis in Streptomyces coelicolor

Paper ID Volume ID Publish Year Pages File Format Full-Text
35291 45084 2009 5 PDF Available
Title
A putative secreted solute binding protein, SCO6569 is a possible AfsR2-dependent down-regulator of actinorhodin biosynthesis in Streptomyces coelicolor
Abstract

AfsR2 is a global regulatory protein involved in stimulating the biosynthesis of antibiotics in various Streptomyces species. Using 2-D gel electrophoresis and MALDI-TOF analyses, several putative AfsR2-target proteins were previously identified. In order to verify the biological significance of the proteomics-guided targets, the sixteen putative AfsR2 target genes were individually cloned under the strong-and-constitutive promoter (PermE*) of the Streptomyces expression vector, pSE34, followed by the transformation into both Streptomyces lividans and Streptomyces coelicolor. Among the putative AfsR2-target genes tested, the expression of a putative secreted solute binding protein, SCO6569 significantly repressed biosynthesis of actinorhodin in S. coelicolor, and the transcripts encoded by the pathway-specific activator, actII-ORF4 for actinorhodin were also reduced in SCO6569-overexpressing S. coelicolor. Moreover, SCO6569 gene-disruption in S. coelicolor further enhanced actinorhodin production, suggesting that the SCO6569 could be a possible AfsR2-dependent down-regulator acting in actinorhodin biosynthesis in S. coelicolor.

Keywords
SCO6569; AfsR2; Actinorhodin; Down-regulator; Streptomyces
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A putative secreted solute binding protein, SCO6569 is a possible AfsR2-dependent down-regulator of actinorhodin biosynthesis in Streptomyces coelicolor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 3, March 2009, Pages 373–377
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us