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Propionic acid production from glycerol by metabolically engineered Propionibacterium acidipropionici

Paper ID Volume ID Publish Year Pages File Format Full-Text
35300 45085 2009 6 PDF Available
Title
Propionic acid production from glycerol by metabolically engineered Propionibacterium acidipropionici
Abstract

Large amounts of crude glycerol produced in the biodiesel industry can be used as a low-cost renewable feedstock to produce chemicals and fuels. Compared to sugars (sucrose, glucose, xylose, etc.), glycerol has a lower reducing level, which is of benefit to the production of reduced chemicals. In this work, glycerol as the sole carbon source in propionic acid fermentation by metabolically engineered Propionibacterium acidipropionici (ACK-Tet) was studied. It was found that the adapted ACK-Tet mutant could use glycerol for its growth and produced propionic acid at a high yield of 0.54–0.71 g/g, which was much higher than that from glucose (∼0.35 g/g). In addition, the production of acetic acid in glycerol fermentation was much less than that from glucose. Thus, glycerol fermentation produced a high purity propionic acid with a high propionic acid to acetic acid ratio of 22.4 (vs. ∼5 for glucose fermentation), facilitating the recovery and purification of propionic acid from the fermentation broth. The highest propionic acid concentration obtained from glycerol fermentation was ∼106 g/L, which was 2.5 times of the highest concentration (∼42 g/L) previously reported in the literature.

Keywords
Propionic acid; Propionibacterium acidipropionici; Glycerol fermentation; Fibrous-bed bioreactor; Metabolic engineering; Adaptation
First Page Preview
Propionic acid production from glycerol by metabolically engineered Propionibacterium acidipropionici
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 12, December 2009, Pages 1346–1351
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering