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Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity

Paper ID Volume ID Publish Year Pages File Format Full-Text
35306 45086 2008 7 PDF Available
Title
Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity
Abstract

Magnetic microspheres were prepared by suspension polymerization of glycidyl methacrylate, ethylene glycol dimethacrylate and vinyl acetate in the presence of oleic acid-coated magnetite, and then, alcoholysed to improve their hydrophilicity. Candida rugosa lipase was covalently immobilized on the hydrophilic magnetic microspheres via the active epoxy groups with the activity yield up to 64.2%. The resulting immobilized lipase had better resistance to pH and temperature inactivation in comparison to free lipase, the adaptive pH and temperature ranges of lipase were widened, and it exhibited good thermal stability and reusability. The resistance of immobilized lipase against urea denaturation was also improved, and the immobilized lipase retained 40% of the activity in the presence of 5 M urea whereas free enzyme retained only 8% of activity. Kinetic parameters were also determined for both immobilized and free lipase. The Km value for immobilized (1.03 mg/ml) was higher than that of the free lipase (0.52 mg/ml), whereas the Vmax value was smaller for the immobilized lipase.

Keywords
Hydrophilic magnetic microspheres; Suspension polymerization; Candida rugosa lipase; Immobilization
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Characterization of Candida rugosa lipase immobilized onto magnetic microspheres with hydrophilicity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 11, November 2008, Pages 1179–1185
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us