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Mathematical modelling of amino acid resolution catalyzed by l-amino acid oxidases from Crotalus adamanteus and Crotalus atrox

Paper ID Volume ID Publish Year Pages File Format Full-Text
35307 45086 2008 9 PDF Available
Title
Mathematical modelling of amino acid resolution catalyzed by l-amino acid oxidases from Crotalus adamanteus and Crotalus atrox
Abstract

Amino acid resolution was studied in this paper. l-Methionine and dl-methionine were used as substrates for the two snake venom l-amino acid oxidases (from Crotalus adamanteus and Crotalus atrox). Even though these enzymes have similar background, they exhibit differences as well as similarities. It was found that both enzymes are active towards l-methionine as substrate (Vm = 0.78 mmol min−1 g−1) and have similar affinities (KmL-met (C. adamanteus) = 0.19 mM, KmL-met (C. atrox) = 0.25 mM). Both enzymes were inhibited in the presence of 2-oxo  -4-methylthiobutyric acid (Ki2-oxo (C. adamanteus) = 0.83 mM, Ki2-oxo (C. atrox) = 2.04 mM), a reaction product of l-methionine oxidative deamination. l-Amino acid oxidase from C. atrox was found to be inhibited by the substrate as well. l-Amino acid oxidase from C. adamanteus was inhibited by d-enantiomer of l-methionine (KiD-met=1.41 mM). Both enzymes were successful in l-methionine oxidation in the batch reactor (100% conversion). This was not the case in the continuously operated enzyme membrane reactor where enzyme deactivation occurred.

Keywords
l-Amino acid oxidase; Enzyme membrane reactor; Enzyme kinetics; Biocatalysis; Biotransformation; Racemate resolution
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Mathematical modelling of amino acid resolution catalyzed by l-amino acid oxidases from Crotalus adamanteus and Crotalus atrox
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 11, November 2008, Pages 1186–1194
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us