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Extraction of chlorpromazine with a new molecularly imprinted polymer from pig urine

Paper ID Volume ID Publish Year Pages File Format Full-Text
35310 45086 2008 6 PDF Available
Title
Extraction of chlorpromazine with a new molecularly imprinted polymer from pig urine
Abstract

Chlorpromazine is extensively used as a sedative hypnotic in veterinary application, however, improper and over usage of chlorpromazine pose a serious threat to human health and animal production. Due to the difficulty in monitoring trace level of chlorpromazine residues in complicated biological matrices, specific adsorption materials for preconcentration and clean-up of chlorpromazine are indispensable for sample preparation before further determination. In this study, a new molecularly imprinted polymer (MIP) for recognition of chlorpromazine was obtained by bulk polymerization. Then the imprinting effect of MIP was confirmed via chromatographic evaluation by compared with the corresponding non-imprinted polymer (NIP). Furthermore, the adsorption selectivity and capacity analysis revealed that the MIP had high specification for chlorpromazine. Finally, with the optimized protocol of molecularly imprinted solid-phase extraction (MISPE), the target analyte in pig urine at different spiked levels were selectively separated and purified for routine HPLC-UV analysis, with the improved recoveries above 73.3% and the intraday RSD of less than 8.7%, proving that the obtained MIP as specific SPE sorbents for extraction of chlorpromazine is applicable.

Keywords
Molecularly imprinted polymer; Chlorpromazine; Solid-phase extraction; Sample preparation; Pig urine
First Page Preview
Extraction of chlorpromazine with a new molecularly imprinted polymer from pig urine
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 11, November 2008, Pages 1209–1214
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering