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Precursor-directed biosynthesis of new dithiolopyrrolone analogs by Saccharothrix algeriensis NRRL B-24137

Paper ID Volume ID Publish Year Pages File Format Full-Text
35315 45086 2008 9 PDF Available
Title
Precursor-directed biosynthesis of new dithiolopyrrolone analogs by Saccharothrix algeriensis NRRL B-24137
Abstract

The natural dithiolopyrrolone antibiotics, produced during fermentation by Saccharothrix algeriensis NRRL B-24137, are known for their potent antibacterial, antifungal, and anticancer activities. During dithiolopyrrolone biosynthesis, the activated organic acid (acyl-CoA) is incorporated into the pyrrothine nucleus. We investigated the use of precursor-directed biosynthesis (PDB) to create new dithiolopyrrolone analogs by substitution of several organic acids in place of the usual organic acid. No other study on dithiolopyrrolone production by precursor-directed biosynthesis has been reported.The production of four novel dithiolopyrrolones in the presence of benzoic, valeric and cinnamic acids by S. algeriensis was obtained in this study. The mass spectrometry data showed a direct incorporation of added valeric and benzoic acids to form several dithiolopyrrolone analogs. However, addition of all other organic acids did not lead to the production of the corresponding dithiolopyrrolone analogs. We describe here the use of the PDB process for production of four new dithiolopyrrolone analogs, valeryl-pyrrothine, benzoyl-pyrrothine, demethyl-benzoyl-pyrrothine and formoyl-pyrrothine.

Keywords
Precursor-directed biosynthesis; New dithiolopyrrolone analogs; Saccharothrix algeriensis; Organic acids; Antibiotic production; Fermentation
First Page Preview
Precursor-directed biosynthesis of new dithiolopyrrolone analogs by Saccharothrix algeriensis NRRL B-24137
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 11, November 2008, Pages 1244–1252
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering