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Development of a low cost culture medium for sakacin A production by L. sakei

Paper ID Volume ID Publish Year Pages File Format Full-Text
35319 45086 2008 6 PDF Available
Title
Development of a low cost culture medium for sakacin A production by L. sakei
Abstract

MRS is currently the medium of choice for promoting sakacin A production by Lactobacillus sakei (180 a.u./ml): however, this medium is expensive and not applicable for large-scale production. In the present study, a one variable (factor) at a time (OVAT) approach was used to formulate an alternative culture medium.The subsequent full factorial design (25) investigated the influence of ingredients on bacteriocin production and interactions among the ingredients. A series of adaptation steps were used to develop an equation model that allowed for estimation of all first order variables and sakacin production. The formula of the alternative culture medium consisted as follows (g/l): bacto peptone (X1) 10, meat peptone (X2) 8, yeast autolysate (X4) 4, glucose 10, CaCO3 30, inoculum 5% (v/v) and use of deionised water. The cost of the alternative medium was approximately half that of MRS and sakacin A production was increased from 180 to 480 a.u./ml.

Keywords
Sakacin A; L. sakei; Bacteriocin; Experimental design; Model adaptation; Full factorial
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Development of a low cost culture medium for sakacin A production by L. sakei
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 11, November 2008, Pages 1275–1280
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us