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Production of clavulanic acid by Streptomyces clavuligerus in batch cultures without and with glycerol pulses under different temperature conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
3533 174 2012 7 PDF Available
Title
Production of clavulanic acid by Streptomyces clavuligerus in batch cultures without and with glycerol pulses under different temperature conditions
Abstract

Clavulanic acid (CA) is a potent beta-lactamase inhibitor produced by Streptomyces clavuligerus. Like other beta-lactam compounds, CA is chemically unstable at high temperatures. The decomposition of CA during bacterial fermentation reduces its concentration in the broth, resulting in low yields. This study investigated the use of temperature reduction to obtain high CA production during fermentation employing cultures of S. clavuligerus. Simple batch cultures, and batch cultures with glycerol pulses, were performed at different temperatures in shake flasks at 250 rpm, and pH 6.8. Firstly, three batch cultures using glycerol as carbon source were carried out at temperatures of 30 (control), 25, and 20 °C. Next, three batch cultures were carried out with temperature reductions from 30 to 25 °C, 30 to 20 °C, and 25 to 20 °C, after glycerol exhaustion. CA production generally increased in cultures with temperature reduction and glycerol feeding. Maximum CA concentration of 1534.3 mg L−1 was achieved in a culture maintained at a constant temperature of 20 °C, with one pulse of glycerol. Under these conditions, both glycerol uptake rate and CA degradation were low. The results obtained demonstrate the potential of temperature reduction in the optimization of CA production by S. clavuligerus.

► Improvement of the clavulanic acid production. ► Batch cultures with glycerol pulses at low temperature. ► Clavulanic acid production rate and clavulanic acid degradation rate as function of the temperature.

Keywords
Clavulanic acid; Streptomyces clavuligerus; Temperature reduction; Glycerol uptake rate; Product degradation rate
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Production of clavulanic acid by Streptomyces clavuligerus in batch cultures without and with glycerol pulses under different temperature conditions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 69, 15 December 2012, Pages 1–7
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us