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Modeling hexanal production in oxido-reducing conditions by the yeast Yarrowia lipolytica

Paper ID Volume ID Publish Year Pages File Format Full-Text
35339 45087 2009 6 PDF Available
Title
Modeling hexanal production in oxido-reducing conditions by the yeast Yarrowia lipolytica
Abstract

Hexanal produced by cells of a recombinant Yarrowia lipolytica yeast expressing the hydroperoxide lyase (HPL) from green bell pepper fruit was studied under oxido-reducing conditions using the reducing dithiotreitol and oxidizing potassium ferricyanide compounds. The combined effect of pH, linoleic acid 13-hydroperoxides concentration, temperature and oxido-reducing molecules on the hexanal production was studied. Significant positive effects for the hexanal production were found using high concentrations of hydroperoxides (100 mM, 30 g/L). Adding reducing molecules enhanced significantly hexanal production while the oxidizing molecules had an inhibitory effect. Combined effects of 13-hydroperoxides and dithiotreitol were optimised by a central composite design and a model was proposed. Finally, 6 mM (600 mg/L) of hexanal was obtained when 119 mM of 13-hydroperoxides (37 g/L) and 50 mM of dithiotreitol were introduced directly in the biocatalytic medium of the yeast Y. lipolytica.

Keywords
HPL, hydroperoxide lyase; LOX, lipoxygenase; 13-HPO, 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid and 13-hydroperoxy-9Z,11E-octadecadienoic acid; Eh, redox potentialHexanal; Redox potential; Dithiotreitol; Potassium ferricyanide; Yarrowia lipolytica
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Modeling hexanal production in oxido-reducing conditions by the yeast Yarrowia lipolytica
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 9, September 2009, Pages 1013–1018
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us