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Production of 3,6-anhydro-l-galactose from agarose by agarolytic enzymes of Saccharophagus degradans 2-40

Paper ID Volume ID Publish Year Pages File Format Full-Text
35359 45088 2011 6 PDF Available
Title
Production of 3,6-anhydro-l-galactose from agarose by agarolytic enzymes of Saccharophagus degradans 2-40
Abstract

Saccharophagus degradans 2-40 is capable of hydrolyzing agarose, a red macroalgae-derived polymer, into d-galactose and 3,6-anhydro-l-galactose (L-AHG). Its agarase system is receiving much attention because it can be used to produce fermentable sugar from agarose. L-AHG is commercially unavailable and is considered a rare sugar with a high value. In this study, cells grown on agarose, agar or red macroalgae biomass were found to have a significantly higher agarase activity and AHG-generating activity than those grown on glucose or galactose. From agar-grown cells, both the volumetric activities of agarases and AHG generation in the cell-free lysate were much higher than in the extracellular fraction. Based on the analyses of the enzyme reaction products, from the reaction with the crude enzymes from cell-free lysate, neoagarobiose with a degree of polymerization (DP) 2 appeared to be the only major product in the initial reaction period, but sugars with DPs 2, 4 and 6 were found to be all predominantly produced by the extracellular enzymes in the initial reaction period. Quantitative analysis of AHG using gas chromatography–mass spectrometry with a derivatization step was also found to be highly reproducible and reliable. These results will be useful for producing L-AHG as a rare sugar to investigate its metabolic fate and commercial utilization.

Keywords
3,6-Anhydro-l-galactose; Saccharophagus degradans; Agarase; Red macroalgae; Gas chromatography–mass spectrometry
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Production of 3,6-anhydro-l-galactose from agarose by agarolytic enzymes of Saccharophagus degradans 2-40
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 1, January 2011, Pages 88–93
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us