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Extracellular poly (A) specific ribonuclease from Aspergillus niger ATCC 26550: Purification, biochemical, and spectroscopic studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
35366 45088 2011 7 PDF Available
Title
Extracellular poly (A) specific ribonuclease from Aspergillus niger ATCC 26550: Purification, biochemical, and spectroscopic studies
Abstract

Aspergillus niger RNase belonging to RNase T2 family is purified to homogeneity using ammonium sulfate precipitation and anion-exchange chromatography from A. niger. The enzyme is glycosylated monomer with molecule mass of 28.5 kDa. Optimum activity of the enzyme is achieved at pH 3.5 and temperature 55 °C. The enzyme shows broad substrate specificity with poly (A), poly (C), poly (G), poly (U) and yeast RNA. The enzyme is highly stable against various metal ions and organic solvents, thus dictates its applicability in various industries. Two peptide sequences, PGGTLLQTQFWDYDP and TLDSYTALSDAGITPSEDATYK obtained by MS/MS analysis and confirmed by BLAST search, show substantial sequence homology with Aspergillus phoenicis, Aspergillus clavatus and P. merneffei. Multiple sequence analysis of the peptide sequences of purified enzyme shows 97%, 75% and 72% identity with A. phoenicis, A. clavatus and P. merneffei, respectively using Clustal W tool. Spectroscopic studies by absorbance, fluorescence, and circular dichroism reveal that the enzyme has α+β type secondary structure with approximately 29% α-helix, 24% β-sheet and 47% random coil by K2D software.

Keywords
BLAST, basic local alignment search tool; BSA, bovine serum albumin; DEAE, diethylaminoethyl; DMSO, dimethyl sulfoxide; DTNB, 5,5μ-dithiobis (2-nitrobenzoic acid); DTT, dithiotritol; EDTA, ethylene diammine tetraacetic acid; GuHCl, guanidine hydrochloride
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Extracellular poly (A) specific ribonuclease from Aspergillus niger ATCC 26550: Purification, biochemical, and spectroscopic studies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 1, January 2011, Pages 135–141
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us