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Discovery of a novel adenosine 5′-phosphosulfate (APS) reductase from the methanarcheon Methanocaldococcus jannaschii

Paper ID Volume ID Publish Year Pages File Format Full-Text
35369 45088 2011 8 PDF Available
Title
Discovery of a novel adenosine 5′-phosphosulfate (APS) reductase from the methanarcheon Methanocaldococcus jannaschii
Abstract

Here we report the first discovery of adenosine 5′-phosphosulfate reductase (APR), a key enzyme of the sulfate reduction pathway, in the methanarchaeon Methanocaldococcus jannaschii. While the sulfate reduction pathway is present in other organisms, it is not expected to exist in methanarchaea because their habitats often already possess an abundance of reduced sulfur, particularly H2S. However, the gene product of open reading frame (ORF) Mj0973 in M. jannaschii possesses sequence similarities with known APRs and 3′-phosphoadenosine-5′-phosphosulfate reductases (PAPRs) from various organisms. In order to further investigate this ORF, we expressed the gene Mj0973 from M. jannaschii and purified the resulting protein. Kinetic studies revealed that the purified protein is able to reduce APS with Escherichia coli thioredoxin (Trx) supplied as the electron donor, but is unable to reduce PAPS. The apparent Km, Vmax, and kcat/Km values at pH 8.0 and 30 °C were 0.29 μM, 0.079 μM mg−1 min−1, and 299,655 M−1 s−1, respectively. This observation of APR activity strongly indicates the presence of an APS-utilizing sulfate reduction pathway in the methanarchaeon M. jannaschii.

Keywords
Adenosine 5′-phosphosulfate reductase (APR); Methanocaldococcus jannaschii; Sulfate reduction pathway
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Discovery of a novel adenosine 5′-phosphosulfate (APS) reductase from the methanarcheon Methanocaldococcus jannaschii
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 1, January 2011, Pages 154–161
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us