fulltext.study @t Gmail

Ganoderic acid Me induces apoptosis through mitochondria dysfunctions in human colon carcinoma cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
35378 45088 2011 7 PDF Available
Title
Ganoderic acid Me induces apoptosis through mitochondria dysfunctions in human colon carcinoma cells
Abstract

Ganoderma lucidum, a traditional Chinese medicinal mushroom, has been used in Asia for several thousand years for the prevention and treatment of a variety of diseases including cancer. In our previous work, ganoderic acid Me (GA-Me), as one of bioactive triterpene compounds purified from Ganoderma lucidum, induced p53-mediated sub-G1 arrest in human colon cells [1]. The apoptotic mechanism of GA-Me induction on tumor cells is yet unclear and was studied in this work. GA-Me was found to possess remarkable cytotoxicity on HCT-116 human colon carcinoma cells in a dose-dependent manner. Interestingly, the expression of anti-tumor protein p53 in GA-Me treated tumor cells was increased in a time dependent manner. Among the pro-apoptotic proteins, Bax was up-regulated, whereas the expression of Bcl-2 was not significantly changed, thus the ratio of Bcl-2/Bax was decreased. Furthermore, GA-Me reduced mitochondria transmembrane potential, released cytochrome c and increased caspase 3 activity during the induced apoptotic process. Our findings show that the anti-cancer bioactivity of GA-Me was mediated by induced apoptosis, resulting from mitochondrial dysfunctions. Our study also suggests that GA-Me may be a novel promising agent for the treatment of human colon carcinoma cells.

Keywords
Ganoderic acid Me (GA-Me); p53; Apoptosis; Anti-tumor activity; Traditional Chinese medicine (TCM)
First Page Preview
Ganoderic acid Me induces apoptosis through mitochondria dysfunctions in human colon carcinoma cells
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 1, January 2011, Pages 219–225
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us