fulltext.study @t Gmail

Partitioning of invertase from tomato in poly(ethylene glycol)/sodium sulfate aqueous two-phase systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
35379 45088 2011 7 PDF Available
Title
Partitioning of invertase from tomato in poly(ethylene glycol)/sodium sulfate aqueous two-phase systems
Abstract

The aim of this study is to extract and purify tomato (Lycopersicon esculentum) invertase using an aqueous two-phase system (ATPS). The ATPSs are formed by mixing the polymer with a salt and a protein solution. Invertase was extracted by partitioning in ATPS composed of polyethylene glycol (PEG) in the presence of sodium sulfate. The partitioning of the enzyme in ATPSs was studied at 25 °C. The effects of phase composition, molecular mass of the PEG, PEG concentration, salt concentration, pH and neutral salt (KCl) concentration on enzyme partitioning and purification were investigated. The best optimal ATPS condition for the partitioning and purification of invertase was 15% (w/w) PEG 3000, 12% (w/w) Na2SO4 and 5% (w/v) KCl (pH 4.5) which increased the purity by 5.5-fold with the recovered activity of 90%. Therefore, ATPS can be effectively used to recover and purify invertase from tomato fruit.

Keywords
Aqueous two-phase system; Purification; Partitioning; Invertase; Polymer/salt system
First Page Preview
Partitioning of invertase from tomato in poly(ethylene glycol)/sodium sulfate aqueous two-phase systems
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 1, January 2011, Pages 226–232
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us