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New kinetic and thermodynamic approach of one-pot synthesis of chiral alcohol by oxidoreductase from Candida parapsilosis

Paper ID Volume ID Publish Year Pages File Format Full-Text
35380 45088 2011 7 PDF Available
Title
New kinetic and thermodynamic approach of one-pot synthesis of chiral alcohol by oxidoreductase from Candida parapsilosis
Abstract

The stereoinversion process involving two sequential stereoselective oxidation and reduction reactions by oxidoreductases was a typical and important one-pot method for chiral secondary alcohol synthesis which has great potential for industrial application. The process to produce (S)-1-phenyl-1,2-ethanediol with whole cell was chosen as a model reaction to investigate the kinetic and thermodynamic properties of key enzymes which catalyzed the single oxidation and reduction step of the whole reaction. The kinetic data from initial rate experiments in the absence of product prove that both oxidation and reduction reactions follow Theorell-Chance BiBi mechanism. Parameters of Vm and ΔS suggest that the oxidation reaction was thermodynamically unfavorable and the restrictive step of the one-pot process was inhibited at high concentration of substrate PED. To remove the inhibition of substrate, extractive biocatalytic methods were successfully applied to improve the substrate PED concentration from 15 g/l to 50 g/l with high optical purity of 91.6% and yield of 82.5%.

Keywords
Candida parapsilosis; Stereoinversion; (S)-1-phenyl-1,2-ethanediol; Kinetic; Extractive biocatalytic method
First Page Preview
New kinetic and thermodynamic approach of one-pot synthesis of chiral alcohol by oxidoreductase from Candida parapsilosis
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 1, January 2011, Pages 233–239
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering