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Synthesis of galacto-oligosaccharides at very high lactose concentrations with immobilized β-galactosidases from Aspergillus oryzae

Paper ID Volume ID Publish Year Pages File Format Full-Text
35382 45088 2011 8 PDF Available
Title
Synthesis of galacto-oligosaccharides at very high lactose concentrations with immobilized β-galactosidases from Aspergillus oryzae
Abstract

β-Galactosidase from Aspergillus oryzae was immobilized by different methods, being the enzyme immobilized in glyoxyl-agarose selected for the synthesis of galacto-oligosaccharides from lactose at high concentrations. The synthesis was optimized with respect to lactose concentration and enzyme to substrate ratio using lactose conversion into galacto-oligosaccharides as objective function. Optimum values were 54.6% (w/v) lactose and 33 IU g−1 lactose and at such conditions conversion was close to 30%, similar to the one obtained with soluble β-galactosidase and comparable with the best reported values for immobilized β-galactosidases. In the sequential batch production of galacto-oligosaccharides, biocatalyst efficiency was increased by 190% with respect to the free enzyme in solution, and 8500 g of galacto-oligosaccharides per gram of enzyme preparation were produced after 10 batches. This amount can be further increased by considering additional batches up to the point of biocatalyst replacement and is significantly higher than the maximum attainable by using the soluble enzyme.

Keywords
Galacto-oligosaccharides; Prebiotic; Immobilized β-galactosidase; Aspergillus oryzae; Enzyme reactor
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Synthesis of galacto-oligosaccharides at very high lactose concentrations with immobilized β-galactosidases from Aspergillus oryzae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 1, January 2011, Pages 245–252
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us