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Antioxidative and ACE inhibitory activities of protein hydrolysates from the muscle of brownstripe red snapper prepared using pyloric caeca and commercial proteases

Paper ID Volume ID Publish Year Pages File Format Full-Text
35393 45088 2011 10 PDF Available
Title
Antioxidative and ACE inhibitory activities of protein hydrolysates from the muscle of brownstripe red snapper prepared using pyloric caeca and commercial proteases
Abstract

Protein hydrolysates from the muscle of brownstripe red snapper (Lutjanus vitta) prepared using Alcalase or Flavourzyme as the first step with 40% degree of hydrolysis (DH), followed by hydrolysis with pyloric caeca protease (PCP) as the second step for 2 (HAP) and 1 h (HFP), respectively, were prepared and determined for their antioxidative and angiotensin I-converting enzyme (ACE) inhibitory activities. HAP had the higher DPPH and ABTS radical scavenging activity and ferric reducing antioxidant power (FRAP), while HFP showed the higher ferrous chelating activity and ACE inhibitory activity (p < 0.05). Both HAP and HFP were able to retard lipid oxidations in lecithin–liposome and β-carotene–linoleic acid oxidation model systems in dose-dependent manner. HAP and HFP contained 87.36 and 86.55% protein (wet basis), respectively with glutamic acid/glutamine as the major amino acids, followed by aspartic acid/asparagine, lysine, alanine and leucine, respectively. HFP showed a slightly greater efficiency in prevention of lipid oxidation in all systems tested. Antioxidative activities, except DPPH radical scavenging activity, of both HAP and HFP after being subjected to gastrointestinal tract model system (GIMs) increased, suggesting the enhancement of antioxidative activities of both hydrolysates after ingestion.

Keywords
Protein hydrolysate; Antioxidative activity; Gastrointestinal model system; Oxidation model system; Two-step hydrolysis
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Antioxidative and ACE inhibitory activities of protein hydrolysates from the muscle of brownstripe red snapper prepared using pyloric caeca and commercial proteases
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 1, January 2011, Pages 318–327
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us