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Biotransformation of ethyl 2-(2′-nitrophenoxy)acetate to benzohydroxamic acid (D-DIBOA) by Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
35398 45088 2011 7 PDF Available
Title
Biotransformation of ethyl 2-(2′-nitrophenoxy)acetate to benzohydroxamic acid (D-DIBOA) by Escherichia coli
Abstract

Benzohydroxamic acids, such as DIBOA, exhibit interesting biological properties (herbicidal, fungicidal and bactericidal). Recently, the synthesis of DIBOA has been simplified to only two steps. This paper explores the possibility of replacing the second stage in the chemical synthesis of D-DIBOA by a biotransformation using a strain of Escherichia coli and a strain of Serratia marcescens. Biotransformation experiments were carried out for both strains in the presence of different concentrations (0.25, 0.5 and 1 mg/mL) of the precursor (ethyl 2-(2′-nitrophenoxy)acetate) under aerobic and anaerobic conditions. Both strains tolerated the concentrations of precursor investigated here. Higher biotransformation yields were reached for E. coli under aerobic conditions. The UV/vis spectra and 1H/13C spectroscopic data obtained from HPLC-DAD and NMR, respectively, for the compounds obtained in the biotransformation reaction confirmed the presence of D-DIBOA in cultures of E. coli. The maximum yields were obtained in experiments supplemented with 0.5 mg/mL of precursor and these were 20.14 ± 1.87% under aerobic conditions and 8.17 ± 0.94% under anaerobic conditions.

Keywords
Biotransformation; Escherichia coli; Serratia marcescens; Benzohydroxamic acid; Natural product
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Biotransformation of ethyl 2-(2′-nitrophenoxy)acetate to benzohydroxamic acid (D-DIBOA) by Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 1, January 2011, Pages 358–364
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us