fulltext.study @t Gmail

Colorimetric protein quantification in aqueous two-phase systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
35408 45088 2011 5 PDF Available
Title
Colorimetric protein quantification in aqueous two-phase systems
Abstract

The polymer-salt rich environment of aqueous two-phase systems disturbs standard protein quantification methods, like UV measurement or Bradford assay.Therefore, we systematically investigated the influence of high polyethylene glycol and phosphate salt concentrations on the readings of three colorimetric protein assays: Bradford, DC (Bio-Rad) and ninhydrin assay.All three assays are troubled by the aqueous two-phase systems components, but disturbances can be significantly reduced by diluting the samples to 1% PEG and 10% salt. But additionally, the Bradford and the DC assay are strongly dependent on the protein composition, which lead to analytical protein recoveries from 0% to 181% in the current study.Especially for salt-rich fractions with low protein concentration and for measuring analytically problematic proteins like RNase A we therefore suggest a ninhydrin assay, which displays minimal protein-to-protein variation and high sensitivity.

Keywords
ATPS, aqueous two-phase systems; BSA, bovine serum albumin; PEG, polyethylene glycol; RNase A, ribonuclease AProtein quantification; Aqueous two-phase systems; Colorimetric protein assay
First Page Preview
Colorimetric protein quantification in aqueous two-phase systems
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 1, January 2011, Pages 413–417
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us