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Aqueous two-phase poly(ethylene glycol)–poly(acrylic acid) system for protein partitioning: Influence of molecular weight, pH and temperature

Paper ID Volume ID Publish Year Pages File Format Full-Text
35414 45089 2008 7 PDF Available
Title
Aqueous two-phase poly(ethylene glycol)–poly(acrylic acid) system for protein partitioning: Influence of molecular weight, pH and temperature
Abstract

The partitioning behavior of two model proteins in poly(ethylene glycol) (PEG)–poly(acrylic acid) (PAA) aqueous two-phase systems has been studied. The effect of polymer molecular weight, tie line length (TLL), pH, temperature and NaCl concentration on the partitioning of proteins (ovalbumin and myoglobin) of different molecular weights and isoelectric points has been studied. Poly(ethylene glycol) is enriched in the top phase while poly(acrylic acid) is found in the bottom phase. An increased partitioning of proteins to the PEG phase has been observed with increased TLL. The protein partitioning decreases on increasing the molecular weight of PEG and temperature, whereas the partitioning increases with increase in pH and NaCl concentration of the system. The protein partitioning is better for both the model proteins using PEG 4000–PAA systems at 20 °C, pH 8.0 with 1 M NaCl. The percentage yield of extraction for myoglobin and ovalbumin is 95.2% and 87.4%, respectively.

Keywords
Aqueous two-phase systems; Globular proteins; Poly(ethylene glycol); Poly(acrylic acid); Ovalbumin; Myoglobin; Partition coefficient
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Aqueous two-phase poly(ethylene glycol)–poly(acrylic acid) system for protein partitioning: Influence of molecular weight, pH and temperature
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 9, September 2008, Pages 905–911
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us