fulltext.study @t Gmail

Feeding strategies for the enhanced production of recombinant human serum albumin in the fed-batch cultivation of Hansenula polymorpha

Paper ID Volume ID Publish Year Pages File Format Full-Text
35416 45089 2008 7 PDF Available
Title
Feeding strategies for the enhanced production of recombinant human serum albumin in the fed-batch cultivation of Hansenula polymorpha
Abstract

An intermittent feeding strategy in fed-batch cultivation has been widely applied to control the accumulation of overflow toxic metabolites and oxygen limitation. However, successive depletions of carbon source by carbon-limited feeding might induce considerable stress to host cells, such as nutrient starvation, which may evoke the enhanced degradation of secreted recombinant proteins by proteolysis. To prevent this degradation, we evaluated novel nutrient feeding strategies for the production of MOX promoter-driven recombinant human serum albumin in the fed-batch cultivation of Hansenula polymorpha. Compared to conventional feeding strategies such as exponential or intermittent feeding strategy, feeding strategies developed in the present study significantly reduced the degradation of recombinant products and yielded high levels of intact recombinant human serum albumin in fed-batch cultivation.

Keywords
Hansenula polymorpha; MOX promoter; Human serum albumin; Fed-batch fermentation; Feeding strategy; Recombinant protein
First Page Preview
Feeding strategies for the enhanced production of recombinant human serum albumin in the fed-batch cultivation of Hansenula polymorpha
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 9, September 2008, Pages 918–924
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us