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Identification of a ripening-related lipoxygenase in tomato fruit as blanching indicator enzyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
35418 45089 2008 5 PDF Available
Title
Identification of a ripening-related lipoxygenase in tomato fruit as blanching indicator enzyme
Abstract

To learn how the protein stability may be correlated to the nutritional quality in tomato (Solanum lycopersicum Mill.) fruit during processing, a 97-kDa protein closely related to ripening and processing quality was pursued in this study. The 97-kDa protein was separated from tomato fruit, identified as lipoxygenase (LOX) by employing MALDI-TOF mass spectrometry and immunoprecipitation. Using the 97-kDa-specific antibodies to determine thermal stability had shown that the 97-kDa LOX of tomato fruit was undetectable after incubating the extract at 70 °C for 10 min, or heating the fruit at 60 °C for 10 min. The changes in 97-kDa LOX by immunoblotting assay are coordinated with the changes in activity of LOX in the fruit under various conditions. The 97-kDa LOX rather than peroxidase is better correlated to the changes of quality in tomato fruit after blanching treatment. The 97-kDa LOX in different tissues from breaker and red-ripe tomato fruit were found to be maximally accumulated in the pericarp of red-ripe tomato fruit. These results suggested that the 97-kDa LOX in tomato fruit is a ripening-related LOX and can be used as an indicator enzyme in tomato processing.

Keywords
Tomato; Identification; Lipoxygenase; Blanching
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Identification of a ripening-related lipoxygenase in tomato fruit as blanching indicator enzyme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 9, September 2008, Pages 932–936
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us