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Partitioning of biomolecules in aqueous two-phase systems of polyethylene glycol and nonionic surfactant

Paper ID Volume ID Publish Year Pages File Format Full-Text
3542 174 2012 7 PDF Available
Title
Partitioning of biomolecules in aqueous two-phase systems of polyethylene glycol and nonionic surfactant
Abstract

The partitioning of various hydrophobic or hydrophilic amino acids was investigated in aqueous two-phase systems (ATPS) composed of polyethylene glycol (PEG) and nonionic surfactant (Triton X-100 and Tween 80), respectively. The influences of amino acids side chain group, tie line length (TLL) of ATPS, nonionic surfactant type and PEG molecular weight on the partitioning coefficients of amino acids were extensively studied. The results displayed that the hydrophilic amino acid preferred to partition in the top PEG-rich phase, and the hydrophobic amino acid preferred to the bottom surfactant-rich phase. These distribution trends of amino acids in PEG/nonionic surfactant ATPS were more obvious with increasing TLL, while the PEG molecular weight had no significant effect on the amino acids partitioning in ATPS. Moreover, the estimated hydrophobic factor (HF) values of PEG/nonionic surfactant ATPS should be about −10−2 mol/kJ according to the amino acids partitioning, which demonstrated that the bottom surfactant-rich phase was generally more hydrophobic than the top PEG-rich phase. PEG20000/Triton X-100 ATPS had more extensive HF values scope (−0.0038 to −0.0496 mol/kJ) than PEG20000/Tween 80 ATPS (HF −0.0162 to −0.0306 mol/kJ) in the investigated TLL due to the different micelles size and number. The partitioning of model protein lysozyme was predicted in PEG/nonionic surfactant ATPS based on ATPS HF and lysozyme hydrophobicity. The experimental and predicted partitioning coefficients of lysozyme displayed a good consistency in the PEG/nonionic surfactant ATPS. It was shown that the hydrophobic interaction played an important role in both the partitioning of amino acids and the partitioning of the small model protein in PEG/nonionic surfactant ATPS.

► The partitioning of amino acids is investigated in PEG/nonionic surfactant ATPS. ► The hydrophobicity of PEG/nonionic surfactant ATPS are estimated. ► The partitioning of lysozyme in ATPS is further predicted. ► The hydrophobic interaction play an important role in the biomolecules partitioning.

Keywords
Aqueous two-phase system; Nonionic surfactant; Amino acids; Hydrophobic; Lysozyme; Partitioning
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Partitioning of biomolecules in aqueous two-phase systems of polyethylene glycol and nonionic surfactant
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 69, 15 December 2012, Pages 93–99
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us