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A stable trypsin inhibitor from Chinese dull black soybeans with potentially exploitable activities

Paper ID Volume ID Publish Year Pages File Format Full-Text
35426 45089 2008 7 PDF Available
Title
A stable trypsin inhibitor from Chinese dull black soybeans with potentially exploitable activities
Abstract

A dimeric 40-kDa Kunitz-type trypsin inhibitor was isolated from seeds of the Chinese black soybean Glycine max cv. ‘Dull Black’. The purification protocol comprised ion exchange chromatography on Q-Sepharose, SP-Sepharose, and Mono Q, and gel filtration on Superdex 75. The trypsin inhibitor inhibited chymotrypsin, albeit to a lesser extent than it inhibited trypsin. Its trypsin-inhibitory activity was unaffected after exposure to pH 1–14, or to temperatures up to 80 °C. The trypsin inhibitor was inhibited by dithiothreitol in a dose-dependent (from 2.5 to 50 mM) and a time-dependent (from 5 to 120 min) manner. Besides inhibiting serine proteases, the trypsin inhibitor demonstrated additional biological activities including stimulation of nitric oxide production by macrophages. It inhibited HIV-1 reverse transcriptase, cell-free translation and proliferation of liver cancer cells and breast cancer cells, with an IC50 value 9.4, 14, 39 and 70 μM, respectively. However, it did not exhibit antifungal, antibacterial or mitogenic activity.

Keywords
Kunitz-type trypsin inhibitor; Black soybean; Anti-proliferative; Isolation; Characterization; Seeds
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A stable trypsin inhibitor from Chinese dull black soybeans with potentially exploitable activities
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 9, September 2008, Pages 992–998
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us