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Kinetics of phenanthrene degradation by Staphylococcus sp. strain PN/Y involving 2-hydroxy-1-naphthoic acid in a novel metabolic pathway

Paper ID Volume ID Publish Year Pages File Format Full-Text
35428 45089 2008 5 PDF Available
Title
Kinetics of phenanthrene degradation by Staphylococcus sp. strain PN/Y involving 2-hydroxy-1-naphthoic acid in a novel metabolic pathway
Abstract

Staphylococcus sp. strain PN/Y isolated from creosote-contaminated soil, was previously reported to degrade phenanthrene as sole source of carbon and energy. Unlike other phenanthrene degraders, Staphylococcus sp. PN/Y degraded phenanthrene by a novel pathway involving 2-hydroxy-1-naphthoic acid (2H1NA), which was further metabolized by unique meta-cleavage dioxygenase, ultimately leading to TCA cycle intermediates. In the present study, kinetics of phenanthrene degradation and the dynamic fate of the key intermediates, 2H1NA, salicylic acid and catechol were demonstrated. When cells were grown on 50, 100, 200, 500 and 1000 mg l−1 of phenanthrene, the doubling time was 31.55, 30.2, 29.32, 26.84, 25.22 h and the specific growth rate was 0.0314, 0.0331, 0.034, 0.0372 and 0.0396 h−1, respectively. At a concentration of 1 g l−1 of phenanthrene, the maximum accumulation of 2H1NA was found to be 323 mg l−1. In addition, 2H1NA utilized by Staphylococcus sp. PN/Y as sole carbon source, the growth yield at 96 h was 445, 557, 560, 480, 419, 334, 212 and 66 mg of protein (g 2H1NA)−1 when cells were grown on 50, 100, 200, 400, 500, 650, 800 and 1000 mg l−1 of 2H1NA, respectively. Growth was found to be inhibited at initial higher concentration of 2H1NA, which may attributed due to concentration-dependent toxicity of 2H1NA and/or the toxicity of its decarboxylated metabolite, 2-naphthol.

Keywords
Phenanthrene; 2-Hydroxy-1-naphthoic acid; 2-Naphthol; Biodegradation; Staphylococcus sp.; Kinetic study
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Kinetics of phenanthrene degradation by Staphylococcus sp. strain PN/Y involving 2-hydroxy-1-naphthoic acid in a novel metabolic pathway
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 9, September 2008, Pages 1004–1008
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us