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Deciphering butanol inhibition to Clostridial species in acclimatized sludge for improving biobutanol production

Paper ID Volume ID Publish Year Pages File Format Full-Text
3543 174 2012 6 PDF Available
Title
Deciphering butanol inhibition to Clostridial species in acclimatized sludge for improving biobutanol production
Abstract

This study aimed to quantitatively evaluate the correlations between butanol (BtOH) tolerance of solvent-producing bacteria (SPB) and the performance of fermentative butanol production. The toxicity potency of BtOH was revealed to suggest the feasibility of butanol formation with a Clostridial species-dominated bacterial consortium. When the mixed culture was grown on the optimal medium comprising 60 g/L glucose, 0.5 g/L FeSO4·7H2O and 5.13 g/L yeast extract, the maximal tolerant butanol concentration, butanol production, hydrogen production and glucose consumption were ca. 16 g/L, 10.64 ± 0.60 g/L, 4153 ± 815 mL/L and 54.99 ± 1.92 g/L, respectively. Moreover, almost all the dose–response curves representing toxicity potency of butanol on microbial characteristics was nearly identical in order of magnitude. Thus, although generations of by-products during butanol fermentation are interactive, the maintenance of microbial growth capability still plays a crucial role to control the performance of butanol production. The quantitative findings in toxicological terms directly suggest that the BtOH toxicity seemed to be inevitable during BtOH production. In addition, butanol inhibition could be reversibly attenuated by removal of butanol to make it below the critical level (ca. 7.83–9.52 g/L or EC50).

► Effect of butanol toxicity on butanol production by a mixed culture was studied quantitatively. ► Butanol inhibition to cell growth and butanol production was analyzed with dose–response curves. ► Feasibility of butanol formation by a bacterial consortium was evaluated in toxicological terms.

Keywords
Fermentation; Microbial growth; Product inhibition; Butanol tolerance; Dose response assessment
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Deciphering butanol inhibition to Clostridial species in acclimatized sludge for improving biobutanol production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 69, 15 December 2012, Pages 100–105
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us