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Protein isolates from jumbo squid (Dosidicus gigas) by pH-shift processing

Paper ID Volume ID Publish Year Pages File Format Full-Text
35444 45090 2009 4 PDF Available
Title
Protein isolates from jumbo squid (Dosidicus gigas) by pH-shift processing
Abstract

There is a growing interest in adding value to the jumbo squid Dosidicus gigas fishery. This work describes two extraction procedures for processing muscle to obtain protein isolates with suitable functional properties. The effect on muscle protein solubility and protein recovery of combining freezing and grinding raw materials during storage was evaluated. Processes are based on extraction of protein at acid or alkaline pH and subsequent iso-electric precipitation. About 85% of the initial muscle protein was solubilized at pH 3 and 11. Regardless of the pH used for extraction, about 90% of the protein was obtained after precipitation at pH 5.5. The total yield from both procedures was 75%. Treatments during storage did not significantly affect solubility and yield of protein. Wastewater contained negligible amounts of protein and may be reused. Processing by acid and alkaline extraction are feasible alternatives for obtaining protein isolates either from fresh or frozen squid muscle, which is an important consideration when choosing the most appropriate and inexpensive method to scale up this technology.

Keywords
Acid and alkaline solubilization; Frozen/ground; pH shift; Protein recovery; Squid muscle
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Protein isolates from jumbo squid (Dosidicus gigas) by pH-shift processing
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 5, May 2009, Pages 584–587
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us