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Purification of chymotrypsin from bovine pancreas using precipitation with a strong anionic polyelectrolyte

Paper ID Volume ID Publish Year Pages File Format Full-Text
35445 45090 2009 5 PDF Available
Title
Purification of chymotrypsin from bovine pancreas using precipitation with a strong anionic polyelectrolyte
Abstract

The separation of chymotrypsin from a crude filtrate of bovine pancreas homogenate was carried out using precipitation with a commercially available negatively charged strong polyelectrolyte: polyvinyl sulfonate. The zymogen form of chymotrypsin was activated by addition of trypsin (0.01 mg/g homogenate), then, the enzyme was precipitated by polyelectrolyte addition at pH 2.5 in the pancreas homogenate. A stoichiometric ratio of 670 bound molecules of chymotrypsin per polyelectrolyte molecule was found in the non-soluble form of the enzyme–polyelectrolyte complex. The non-soluble complex was separated by simple centrifugation and re-dissolved by a pH change to 8.0. The recovery of chymotrypsin biological activity was 61% of the initial activity in the homogenate with 4.7-fold increase in its specific activity.

Keywords
ChTRP, chymotrypsin; PVS, polyvinyl sulfonate sodium saltChymotrypsin; Polyvinyl sulfonate; Protein precipitation
First Page Preview
Purification of chymotrypsin from bovine pancreas using precipitation with a strong anionic polyelectrolyte
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 5, May 2009, Pages 588–592
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering