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Enzyme stability and stabilization—Aqueous and non-aqueous environment

Paper ID Volume ID Publish Year Pages File Format Full-Text
35450 45091 2008 14 PDF Available
Title
Enzyme stability and stabilization—Aqueous and non-aqueous environment
Abstract

Enzyme stabilization has notable importance due to increasing number of enzyme applications. Stabilization of enzymes in order to realize their full potential as catalysts is discussed in the present review. An overview of the denaturation mechanisms in aqueous and non-aqueous environment is given. Further various methods of enzyme stabilization with respect to their use in aqueous and non-aqueous environment have been given. Using thermophilic enzymes as the reference point, a review of stabilization using various approaches like protein engineering, chemical modifications of enzymes and immobilization has been attempted. Finally, it has been stressed that, for selection of a suitable stabilization approach the intended use and possible interactions between the stabilizer-enzyme have to be taken into consideration.

Keywords
Enzyme; Stability; Immobilization; Reverse micelle; Surfactant coating; Chemical modification; CLEAs
First Page Preview
Enzyme stability and stabilization—Aqueous and non-aqueous environment
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 10, October 2008, Pages 1019–1032
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering