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Purification and characterization of lipase from solvent tolerant Pseudomonas aeruginosa PseA

Paper ID Volume ID Publish Year Pages File Format Full-Text
35452 45091 2008 7 PDF Available
Title
Purification and characterization of lipase from solvent tolerant Pseudomonas aeruginosa PseA
Abstract

Lipase from a solvent tolerant strain of Pseudomonas aeruginosa PseA has been purified by gel exclusion chromatography leading to 8.6-fold purification and 51.6% recovery. Mr of purified lipase as determined by SDS–PAGE was estimated to be approximately 60 kDa. The optimum pH and temperature for activity of lipase were found to be 8.0 and 40 °C. The lipase was found to be stable in the pH range 6–8.5 and temperature range 25–50 °C. It was stable in presence of divalent metal ions like Ca2+, Mg2+ whereas Cu2+ and Zn2+ were found to be inhibitory. The enzyme activity was not affected significantly by 1 mM EDTA. β-Mercaptoethanol reduced the enzyme activity to 48% after 1 h whereas glutathione activated the lipase. Serine inhibitor PMSF showed no reduction in enzyme activity. Non-ionic detergents Tween-80 and Brij-35 stimulated the lipase activity. Cationic surfactant CTAB inhibited the enzyme activity whereas anionic surfactants sodium deoxycholate caused only 10% reduction in activity. Lipase preferred longer carbon chain (C16) fatty acid ester substrates over the shorter ones and showed random positional specificity for triolein hydrolysis.

Keywords
Pseudomonas aeruginosa PseA; Solvent tolerant; Lipase; Purification; Characterization; Positional specificity
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Purification and characterization of lipase from solvent tolerant Pseudomonas aeruginosa PseA
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 10, October 2008, Pages 1040–1046
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us