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Optimization of bacteriorhodopsin production by Halobacterium salinarium PTCC 1685

Paper ID Volume ID Publish Year Pages File Format Full-Text
35457 45091 2008 6 PDF Available
Title
Optimization of bacteriorhodopsin production by Halobacterium salinarium PTCC 1685
Abstract

This paper is concerned with medium optimization for the production of bacteriorhodopsin (BR) by Halobacterium salinarium PTCC 1685 by one-factor-at-a-time and Taguchi's array methods. The one-factor-at-a-time method was used to study the effects of various carbon and nitrogen sources, temperature and initial pH on bacteriorhodopsin production. Among these variables, corn steep powder, universal peptone and yeast extract were the most suitable sources, respectively. The optimal temperature and initial pH for bacteriorhodopsin production were 38 °C and 7.5, respectively. Subsequently, the concentration of corn steep powder and other medium components were optimized using Taguchi's method. The results showed that corn steep powder, glycerol and meat extract had significant effect on bacteriorhodopsin production by H. salinarium PTCC 1685. The optimal concentrations of nutritional components for improved bacteriorhodopsin production were determined as 10 g l−1 meat extract, 3.75 g l−1 casamino acids, 10 g l−1 glycerol, 50 g l−1 corn steep powder, 1 g l−1 Na3-citrate, 6 g l−1 KCl, 0.2 g l−1 FeSO47H2O, 0.2 g l−1 MnSO47H2O and 10 g l−1 MgSO47H2O. The maximum bacteriorhodopsin production in shake flask culture was 191.7 mg l−1 about 2.85-fold higher than those obtained with the basal medium. Under optimal culture conditions, the maximum bacteriorhodopsin production in a 15-l stirred-tank bioreactor indicated 234.6 mg l−1 about 3.49-fold higher than the basal medium.

Keywords
Bacteriorhodopsin; Halobacterium salinarium PTCC 1685; Medium components; Optimization; Taguchi's array method; Production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 10, October 2008, Pages 1077–1082
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us