fulltext.study @t Gmail

Purification and partial characterization of a novel β-1,3-glucanase from the gut of sea cucumber Stichopus japonicus

Paper ID Volume ID Publish Year Pages File Format Full-Text
35461 45091 2008 5 PDF Available
Title
Purification and partial characterization of a novel β-1,3-glucanase from the gut of sea cucumber Stichopus japonicus
Abstract

For the first time, a β-1,3-glucanase was isolated from the gut of sea cucumber. The enzyme was purified to homogeneity by ammonium sulfate precipitation, ion exchange chromatography with diethylaminoethyl (DEAE)-Sepharose CL-6B, hydrophobic interaction chromatography with Butyl-Sepharose and preparative electrophoresis with polyacrylamide gel electrophoresis (PAGE). The molecular weight of the purified enzyme was estimated to be 37.5 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme exhibited optimum activity at pH 5.5 and 40 °C. The enzyme showed high pH stability within the range of pH 5.0–8.0 and thermostability up to 40 °C. The enzyme activity was markedly activated by Mn2+, whereas strongly inactivated by Cu2+ and Ag+. The Km and Vmax of the β-1,3-glucanase were 19.8 μg ml−1 and 2000 μg min−1 mg−1, respectively. The amino acid sequences of three segments of the purified enzyme were acquired by mass spectrometer method, which did not have any homology with previously described echinoderm β-1,3-glucanases, suggesting that it may be a novel member of echinoderm β-1,3-glucanase.

Keywords
Purification; Characterization; Sea cucumber (Stichopus japonicus); β-1,3-Glucanase; Amino acid sequences; Digestive enzymes
First Page Preview
Purification and partial characterization of a novel β-1,3-glucanase from the gut of sea cucumber Stichopus japonicus
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 10, October 2008, Pages 1102–1106
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us