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Production and characterization of a novel antimicrobial peptide HKABF by Pichia pastoris

Paper ID Volume ID Publish Year Pages File Format Full-Text
35465 45091 2008 8 PDF Available
Title
Production and characterization of a novel antimicrobial peptide HKABF by Pichia pastoris
Abstract

HKABF, Hippocampus kuda antibacterial factor, is a putative antimicrobial peptide identified from the cDNA library of the brooding pouch of H. kuda Bleeker. The putative mature peptide, fused with a 6His-tag, an anionic peptide (SAPLNPDNID) and to an enterokinase cleavage site was expressed in Pichia pastoris. The mature HKABF peptide, released by enterokinase cleavage, showed antimicrobial activity against Staphylococcus aureus and Staphylococcus saprophyticus at low concentrations (0.5–2.5 μg/ml). The recombinant mature peptide was heat-stable and had low hemolytic activity. Circular dichroism (CD) spectroscopy data indicates its classification as a CSαβ-type antimicrobial peptide. This study serves as an impetus for the production and further research of this antimicrobial peptide.

Keywords
Antimicrobial peptide; HKABF; Recombinant expression; Purification; Pichia pastoris; Antimicrobial activity
First Page Preview
Production and characterization of a novel antimicrobial peptide HKABF by Pichia pastoris
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 10, October 2008, Pages 1124–1131
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering