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Reversible immobilization of a hexameric α-galactosidase from Thermus sp. strain T2 on polymeric ionic exchangers

Paper ID Volume ID Publish Year Pages File Format Full-Text
35468 45091 2008 5 PDF Available
Title
Reversible immobilization of a hexameric α-galactosidase from Thermus sp. strain T2 on polymeric ionic exchangers
Abstract

Agarose coated with polyethyleneimine (PEI) or sulfate-dextran, monoaminoethyl-N-aminoethyl-agarose, DEAE-agarose and carboxymethyl-agarose was used to immobilize by ionic exchange and α-galactosidase from Thermus sp. T2 under different conditions. Supports coated with polymers (PEI or sulfate-dextran) allowed higher immobilization yields (by 10–20%) than mono-functional supports. Moreover, anionic exchangers permitted higher immobilization yields and expressed activities when compared to the cationic exchangers (98% or 67%, respectively under optimal conditions). Curiously, the enzyme could be immobilized on both anionic and cationic exchangers under identical conditions. Both MANAE and PEI-coated supports immobilized the enzyme at pH 7 and gave the highest immobilization yield, expressed activity (over 95%), stability and adsorption strength.Adsorption of the enzyme on PEI-coated supports allowed to greatly increase the enzyme stability at pH 5, 7 and 9 (by over 600-1000 folds), improving the activity at temperatures over the optimal (e.g., at 90 °C the free enzyme retained less than 10% of the maximal activity, while the immobilized enzyme retained almost 60%) and at alkaline pH values (e.g., the immobilized enzyme was 4-fold more active at pH 9 than the free enzyme).

Keywords
Thermus sp. strain T2; α-galactosidase; Polyethyleneimine supports; Reversible immobilization of proteins; Stabilization of proteins
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Reversible immobilization of a hexameric α-galactosidase from Thermus sp. strain T2 on polymeric ionic exchangers
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 10, October 2008, Pages 1142–1146
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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