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Carotenoid content in mutants of the chlorophyte Chlamydomonas reinhardtii with low expression levels of phytoene desaturase

Paper ID Volume ID Publish Year Pages File Format Full-Text
35469 45091 2008 6 PDF Available
Title
Carotenoid content in mutants of the chlorophyte Chlamydomonas reinhardtii with low expression levels of phytoene desaturase
Abstract

More than 90% of the carotenoids world market corresponds to chemical synthesis, but the growing demand of natural additives makes their production from microorganisms very attractive. Microalgae are one of the main sources of natural carotenoids. Although there is extensive information about the main pathways involved in the synthesis of carotenoids, the control mechanisms for this route are not clear yet. Phytoene desaturase is a key enzyme in the carotenogenic pathway. Its downregulation by homologous recombination is not possible in microalgae, in which exogenous genes are randomly inserted in the nuclear genome. Post-transcriptional silencing by antisense or inverted-repeat constructions has helped to the functional characterization of a good number of genes. Here, we show that transformation of Chlamydomonas reinhardtii with DNA encoding short hairpin RNA sequences targeting phytoene desaturase and with an antisense pds cDNA fragment causes underexpression of the target gene, but interestingly does not influence on the carotenoid profile.

Keywords
pds; RNA interference; Carotenoids; Microalgae; Chlamydomonas reinhardtii
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Carotenoid content in mutants of the chlorophyte Chlamydomonas reinhardtii with low expression levels of phytoene desaturase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 10, October 2008, Pages 1147–1152
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us