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Dynamic organization of replication forks into factories in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
35473 45091 2008 7 PDF Available
Title
Dynamic organization of replication forks into factories in Escherichia coli
Abstract

In rapidly growing bacteria, replication cycles overlap and the cells contain replisomes assembled at different generations. We have compared the organization of replication forks in cultures with different cell cycles, strains and labeling techniques. The extent of fork-colocalization was found to be dependent on the replication pattern. In slowly growing cells, sister forks were found to colocalize for a fraction of the replication time, whereas cells growing with higher overlapping of replication cycles showed an extensive grouping of replication forks. These results indicate that clustering of replisomes correlates with the number of rounds of replication harbored by each chromosome. The dynamic organization of replisomes reported here, may allow ending the controversy among alternative models. Cell division, DNA-to-cell mass ratio and the integrity of the replication factories were compromised in a ribonucleotide reductase mutant. These results reinforce the idea that regulatory networks connect the synthesis of DNA precursors with the cell cycle.

Keywords
Replication factory; Bacterial cell cycle; Escherichia coli; Replication fork; Replication foci; Nucleotide synthesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 10, October 2008, Pages 1171–1177
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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