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A stable immobilized d-psicose 3-epimerase for the production of d-psicose in the presence of borate

Paper ID Volume ID Publish Year Pages File Format Full-Text
35479 45092 2009 7 PDF Available
Title
A stable immobilized d-psicose 3-epimerase for the production of d-psicose in the presence of borate
Abstract

Maximal activity of the immobilized d-psicose 3-epimerase from Agrobacterium tumefaciens on Duolite A568 beads was achieved at pH 9.0 and 55 °C with borate, and at pH 8.5 and 50 °C without borate. The half-lives of the immobilized enzyme at 50 °C with and without borate were increased 4.2- and 128-fold compared to that of the free enzyme without borate, respectively. The immobilized enzyme with borate produced 441 g l−1 psicose from 700 g l−1 fructose at pH 9.0 and 55 °C, whereas 193 g l−1 psicose was produced without borate at pH 8.5 and 50 °C after 120 min in a batch reaction. The immobilized enzyme in a packed-bed bioreactor without borate was produced continuously 325 g l−1 psicose from 500 g l−1 fructose at a dilution rate of 1.62 h−1 over a 236 h period with productivity of 527 g l−1 h−1 while that without borate produced 146 g l−1 psicose at 4.15 h−1 over a 384-h period with productivity of 606 g l−1 h−1. The operational half-lives of the enzyme with and without borate in the bioreactor were 601 and 645 h, respectively. In the present study, psicose was produced stably with high productivity using the immobilized d-psicose 3-epimerase in the presence of borate.

Keywords
d-Psicose 3-epimerase; Immobilization; Packed-bed reactor; Borate; Agrobacterium tumefaciens; D-Psicose
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A stable immobilized d-psicose 3-epimerase for the production of d-psicose in the presence of borate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 8, August 2009, Pages 822–828
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us