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A multi-level meta-heuristic algorithm for the optimisation of antibody purification processes

Paper ID Volume ID Publish Year Pages File Format Full-Text
3548 174 2012 11 PDF Available
Title
A multi-level meta-heuristic algorithm for the optimisation of antibody purification processes
Abstract

Multi-product biopharmaceutical facilities need flexible process configurations that can adapt to products with diverse characteristics and impurity loads so as to avoid bottlenecks and delays, whilst meeting final product specifications and cost targets. In order to aid the design of such facilities, this work presents a meta-heuristic optimisation approach using genetic algorithms where different levels of decision are addressed (facility, product sequence and unit operation) and multiple process and business criteria are used to evaluate each alternative generated. This is applied to a case study on the production of therapeutic monoclonal antibodies (mAbs), with a focus on the optimal purification sequences and chromatography column sizing strategies to cope with different facility configurations of upstream and downstream trains and different product impurity loads. The industrial case study provides novel insights that allow the identification of the most cost-effective purification sequences and column sizing strategies that meet demand and purity targets for each product in the facility. Emphasis is placed also on providing methods to visualise the trade-offs in the set of optimal solutions with similar cost values so as to enhance the decision making process.

► A decisional tool to optimise biopharmaceutical purification was created. ► An industrial case study on the production of therapeutic antibodies was addressed. ► Chromatography sequences were optimised to meet cost and impurity removal targets. ► Genetic algorithms optimised column sizing strategies to meet time constraints. ► Graphical tools visualised user preferences for selecting alternative strategies.

Keywords
DSP, downstream processing; GA, genetic algorithm; mAb, monoclonal antibody; PBC, packed-bed chromatography; USP, upstream processing; USP:DSP, ratio of upstream to downstream trainsBiopharmaceutical manufacturing processes; Antibody purification; Process
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A multi-level meta-heuristic algorithm for the optimisation of antibody purification processes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 69, 15 December 2012, Pages 144–154
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us