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Cell physiology and metabolic flux response of Klebsiella pneumoniae to aerobic conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
35485 45092 2009 7 PDF Available
Title
Cell physiology and metabolic flux response of Klebsiella pneumoniae to aerobic conditions
Abstract

Cell physiology and metabolic flux distribution of Klebsiella pneumoniae under anaerobic, micro-aerobic and sufficient aerobic conditions were compared. Comparing with the anaerobic condition, the carbon flux flowed from glycerol to biomass increased 10.1% and 389.9%, while the flux flowed to 1,3-propanediol decreased 10.3% and 92.9% under micro-aerobic and sufficient aerobic conditions, respectively. Furthermore, the carbon flux flowed to TCA cycle increased 5.9% and 31.0% under such two conditions. The energy analysis results revealed that the oxygen was favorable for the NADH2 synthesis, but excessive oxygen was disadvantage for the NADH2 utilization in 1,3-propanediol synthesis process. So, the aeration control is significant for the aerobic 1,3-propanediol fermentation. This work is considered helpful for the further understanding of the glycerol metabolism by Klebsiella pneumoniae under aerobic condition and to establish a rational aeration control strategy for 1,3-propanediol aerobic fermentation in a large-scale bioreactor.

Keywords
Klebsiella pneumoniae; 1,3-Propanediol; Metabolic flux analysis; Cell physiology; Aerobic fermentation; Aeration control
First Page Preview
Cell physiology and metabolic flux response of Klebsiella pneumoniae to aerobic conditions
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 8, August 2009, Pages 862–868
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering