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Enhanced biotransformation of l-phenylalanine to 2-phenylethanol using an in situ product adsorption technique

Paper ID Volume ID Publish Year Pages File Format Full-Text
35489 45092 2009 5 PDF Available
Title
Enhanced biotransformation of l-phenylalanine to 2-phenylethanol using an in situ product adsorption technique
Abstract

An in situ product adsorption technique was used to enhance the biotransformation of l-phenylalanine to 2-phenylethanol by Saccharomyces cerevisiae BD. As a suitable adsorbent, the non-polar macroporous resin D101, selected from several resins tested, showed high adsorption capacity for 2-phenylethanol but not l-phenylalanine. Product inhibition was effectively alleviated by the addition of macroporous resin D101 to the biotransformation medium. When 2 g of hydrated resin D101 was added to 30 mL of the biotransformation medium, the total 2-phenylethanol concentration achieved was 6.17 g/L, of which 3.15 g/L remained in the aqueous phase and 3.02 g/L was adsorbed onto the resin. The molar yield of 2-phenylethanol reached 0.70 after 24 h cultivation. Addition of the macroporous resin greatly increased the volumetric productivity of 2-phenylethanol, and made the downstream processing more feasible and easier to perform in an industrial application.

Keywords
2-Phenylethanol; Flavor; Biotransformation; Saccharomyces cerevisiae; In situ product adsorption; Macroporous resin
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Enhanced biotransformation of l-phenylalanine to 2-phenylethanol using an in situ product adsorption technique
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 44, Issue 8, August 2009, Pages 886–890
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us